Immobilized metal ion affinity chromatography sorbents were prepared for purification of reduced glutathione (GSH) using chitosan as substrate.
The expressed protein was purified by one-step affinity chromatography, and its ability of binding plasmid was investigated via gel retardation experiments.
Furthermore, the protein isolated in the periplasm can be purified by nickel ion affinity chromatography.
Purification of recombinant lysostaphin by monoclonal antibody affinity chromatography