By G418 screening and colonies counting, gene pool A was chosen for the second cycle transfection.
經G418篩選並計集落數,選擇克隆數最多的基因池進入第二輪。 如此反覆進行。
採用較低的細胞密度鋪板,加入含最小致死量G418的DMEM培養基進行再次篩選;
用新黴素類似物(G418)篩選出陽*克隆,並用流式細胞術進一步篩選出高效表達克隆,建立穩定高效表達CHO細胞株。
The positive clones were selected by G418, and highly expressing clones were more selected by flow cytometry, and get stable highly expressing CHO cell lines.
The cells were planked with lower cell density, and further screened in DMEM medium containing G418 MFD;