The paper deals with the researches and importance of micro-satellite primers shared by different plant species and genera.
Used as a topcoat over suitable primers. Excellent for use in offshore, Industry, ships, bridges, hydropower and other heavy Industry.
Differences were found among 8 strains from RAPD analysis. Out of 23 random primers, 11 were polymorphic. Altogether 59 RAPD bands were found and the polymorphic rate was 47.5%.
Children learn knowledge from primers.
The specificity of two primers were verified very well by nested-PCR, and they can be used as molecular probes.
Random Amplified Polymorphic DNA( RAPD) analysis was conducted with 5 random paired primers in protoplasted homokaryons and hybrid heterokaryons of Agaricus bisporus in this study.
ISSR analysis with 18 primers to 12 wax apple(Syzygium samarangense Merr. et Perry)germplasm and 2 closely related species produced 459 unambiguous bands, out of them 435(94.77%) were polymorphic.
HLA-DR,-DQ typing by PCR amplification with sequence-specific primers(PCR-SSP);
The BOV97M and bovine 1.715 satellite DNA sequences were selected as the primers of male and bovine-specific DNA, respectively.
Using the specific primers REA-FEA designed in this work by ourselves, we proved the specificity and sensitivity of the immuno-capture-PCR method again. The same result has been achieved.
The authors considered:(1)To design primers attention must be paid to evade UGA and to take place of UGG.
Microsatellite DNA primers were used to analyse whether there were some quantitative trait loci or main gene affecting egg quality and production traits in New Yangzhou chicken populations.
The primer design is a key step for RAMP and REMAP, in which anchored simple sequence repeat primers were used.
Designed the primers of the other 15 new microsatellites, and 10 primers could obtain targeted fragments by PCR amplification.
After DNA elongation, such molecules can serve as a template for amplification and will be picked up in the final product, as will product generated by non-specific annealing of PCR primers.
Because of their excellent adhesion to metals, epoxies are widely used as primers.
Both the 93 polymorphic loci and the 31 polymorphic primers might provide a reliable molecular marker system for grass carp genomic DNA polymorphism analysis.
EST-SSR primers were used to identify the male parents for Yulv and Yusun among Yabukita seeding and other 4 possible male parents.
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