And the contents of total alkaloid in adventitious roots is higher than that in calli and buds.
The calli produced buds on differentiation medium.
This is the first report of obtaining bergenin from calli of Ardisia spp.
These young stems as explants placed on different media formed calli, whose amount then increased by different cultures on different media and for different generations.
The bud can easily produce calli and propagate but has no differentiation on the culture medium.
They were induced to small calli in the medium.
Toxin has effect on growth and differentiation of calli.
1. The calli of different explant from the same variety varied when induced, among which Burgeons were more apt to induced into laxy and transparent calli.
The analysis result showed that the contents of total alkaloids in hairy roots were higher than explants and calli.
Results The calli treated with 0.3% EMS grows faster, contains higher content of total indole alkaloids than the contrast.
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